Automated Counting Cells in 1000 slides Licensed to Researchers

Technology Solutions for RESEARCH

 ‘Virtual’ flow cytometry: objective counting of cells licensed to JMC Surgery ENT Dr. Luginbuhl for easy counting of stromal cells

A new method could provide an important quantitative tool for research scientists and clinical researchers who need a tool to count cells in an automated rapid and objective manner.

 Recently, a medical student interning with Dr. Adam contacted IHCFLOW and presented to us a problem common in research facilities: counting immunostained cells in about a thousand of microscopic slides. Not daunted by the task of manually counting, the student nevertheless ask the question, is there a better way to go through the slides in a systematic, objective, real time operation short of manually drawing grids on the glass slides and counting cells inside the grids. They were interested in counting Heme Oxidase and VEGF positive cells, see below for some results.

On Feb 21, 2012, he sent us this email:

“My name is Giuseppe and I am a medical student researching at Jefferson Medical College. I am looking for software solutions to analyzing the cell number on virtual slides stained with IHC . Can your product analyze cells stained with H&E, and with DAB staining? I need a simple positive cell count. Do you have a trial software that I could use to see if it would work on my slides. I have 1000 (approx) slides that need to be analyzed for a cell count. There is some variance in terms of background and over-staining.

He sent us 4 images to play with initially and subsequently we trained the virtual Flow with more images and a sample data validation with images shown below after several iteration of custom software training and revalidations. After validation, the department acquired the license to use the technology for its research on July 2012.

Product: Auto Stroma1 and Auto Stroma2

OTHERS

AUTOIMMUNOFLUOR DEMO 

FOR RESEARCH STUDIES TO QUANTIFY INTRACELLULAR ANTIGEN DENSITY PER CELL BASIS USING  STAINED CELLS IN TISSUE CULTURE MARKED WITH DIFFERENTIAL NUCLEAR DYE AND CYTOPLASMIC DYE

OTHER PRODUCTS AND MODULES AVAILABLE FOR RESEARCH USE:

AUTO RETICULIN  (Measure  BONE MARROW FIBROSIS by rapid automated computerized algorithm. Just open your reticulin stained color jpeg image, and voila! you get the grade (none to severe (3-4+). For demo, click on this link for animation video.